#Protein ladders for western blotting verification
#Protein ladders for western blotting plus

~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDaĮach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainĬolorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsĬolorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. ~1236, 1048, 720, 480, 242, 146, 66, 20 kDa Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. ~10.7, 9.5, 8.3, 8.0, 7.8, 7.4, 6.9, 6.0, 5.3, 5.2, 4.5, 4.2, 3.5 Thermo Scientific PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5 to 250 kDa) for use as size standards in protein electrophoresis. Protein size estimation and Western transfer monitoring with highQu prestained protein ladder Coloured & sharp protein bands Room temperature stable. Peppermint Stick Phosphoprotein Molecular Weight StandardsĬand圜ane Glycoprotein Molecular Weight Standards Tagged- proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceĭirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) Tagged-proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence After transferring the protein bands onto the blot paper, stain the paper with Ponceau-S. PageRuler Unstained Broad Range Protein LadderĪccurate estimation across a broader range If you want to do with unstained ladder yet, I recommend to do all steps of western blot just routinely do.

Recommended Volume per well for 1.0 mm gel (μL)Ĭolorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands)

#Protein ladders for western blotting plus

Visible monitoring of gel separation and transfer efficiency Precision Plus Protein WesternC Standards Standard plugs are offered for 2-D electrophoresis applications: Precision Plus Protein Unstained Standard Plugs, Strep-tagged, recombinant, pkg of 24 Related Categories.

#Protein ladders for western blotting verification

However this is typically a result of unspecific hybridisation.Spectra Multicolor Broad Range Protein Ladderīest multicolor prestained ladder for routine applicationsĤ colors for improved visualization during separation and transferĪnalysis of high molecular weight proteins Our Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on. Due to high amount of the protein in each ladder band, the ladder might show positive results in immunodetection of blots.Use a bit less of the ladder (2-3 µl) for Western blots.Take 3-5 µl of the ladder for a small size gel slot for electrophoresis.Room-temperature-stable ladders, always ready to use.Can I use your prestained standards for native gel electrophoresis Our protein standards are not designed for protein quantitation. Prestained Protein Ladders Unstained Protein Ladders Western Blotting Protein Standards IEF and 2D Electrophoresis Protein Standards Resources Pick the Best Standards for Your Blots Just click on the light source in our interactive guide and see how banding pattern changes with light source. Monitoring of electrophoresis process and Western transfer efficiency Prestained ladders may be used for molecular weight approximation, confirming gel migration and estimating blotting efficiency but for accurate molecular weight estimation, an unstained ladder should be used.Approximate protein molecular weight determination on denaturing gels.– Research Fellow, Imperial College London We could use as little as 5µl which makes these markers cost effective. The ladders also transfer well to membranes for use in Western Blotting and show up clearly in our Biorad imager. I have struggled to find markers which go over 250kDa of high quality and the CozyXL is by far the best one I have used so far. Use the ladder for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly. Includes 11 protein bands from 8 to 260 kDa (see figure). It is used as protein size markers in immuno-detection in. The ladders give nice distinct bands and stain very well using silver stain, not at all blurry and overstained which some of the ladders from market leaders do. The Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection. The dual function Western Protein Marker is a ready-to-use mixture of ten IgG-binding proteins. We have recently bought and tested the CozyHi and CozyXL protein ladders for SDS-PAGE.